Journal: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association

Article Title: Glomerular proteins related to slit diaphragm and matrix adhesion in the foot processes are highly tyrosine phosphorylated in the normal rat kidney.

doi: 10.1093/ndt/gfp697

Figure Lengend Snippet: Fig. 9. SHPS-1 is predominantly localized in the podocyte of glomerulus. (A) Double-labeled immunofluorescence microscopy using anti-SHPS-1 and anti-synaptopodin antibodies. Frozen sections of normal rat kidney were fixed by PLP before incubated with the mixture of anti-SHPS-1 and anti- synaptopodin antibodies, then sequentially with FITC-conjugated anti-rabbit IgG and Texas-Red-conjugated anti-mouse IgG. Arrows indicate glomerular capillary walls. (B) Immunoelectron microscopy using anti-SHPS-1 antibody. Ultrathin sections from PLP-perfused kidney were probed with anti-SHPS-1, followed by gold-labeled anti-rabbit IgG. Symbols: P, podocyte; GBM, glomerular basement membrane; E, endothelial cell. Arrows indicate the basal membrane of foot processes, asterisks indicate slit diaphragms and arrowheads indicate the apical membranes of foot processes.

Article Snippet: Secondary goat antibodies used herein were gold-labeled anti-mouse and anti-rabbit IgG (GE Healthcare, Chalfont, St. Giles, UK), FITC-conjugated anti-rabbit IgG (pre-absorbed with rat IgG, Immuno-Biological Laboratories, Gumma, Japan), Texas-Red-conjugated anti-mouse IgG (Rockland Immunochemicals, Gilbertsville, PA, USA), HRP-conjugated anti-mouse and anti-rabbit IgG (DakoCytomation, Hamburg, Germany).

Techniques: Labeling, Immunofluorescence, Microscopy, Incubation, Immuno-Electron Microscopy, Membrane

Journal:

Article Title: The suppression of brain cold-stable microtubules in mice induces synaptic defects associated with neuroleptic-sensitive behavioral disorders

doi: 10.1101/gad.223302

Figure Lengend Snippet: Brain anatomy in STOP−/−, STOP+/−, and wild-type adult mice. (A) STOP distribution in the olfactory bulb (OB), cerebellum (Cb), and hippocampus (Hip) from wild-type mice (wt). Parasagittal brain sections were stained with 23C STOP antibody. (B) Cell layer organization in wild-type (wt) and STOP−/− (−/−) mice, in the brain regions shown in A. Parasagittal brain sections were stained with cresyl-violet. (C) LacZ expression in parasagittal brain sections from heterozygous mice (+/−) and STOP−/− (−/−) mice, revealed by β-galactosidase activity. (D) Barrel field organization of the somatosensory cortex in wild-type (wt) and STOP−/− (−/−) mice. Tangential brain sections were stained to reveal the cytochrome oxidase activity pattern. (E) Mossy fiber organization in wild-type (wt) and STOP−/− (−/−) mice. Staining for zinc by the Timm sulfide-silver method showed similar mossy fiber pathway in wild-type and STOP−/− mice. (F) Dendritic organization of Purkinje cells in wild-type (wt) and STOP−/− (−/−) mice. Cerebellum sections stained for calbindin indicated normal dendritic arborization in STOP−/− mice. (G) Representative examples of CA1 pyramidal cells in wild-type (wt) and STOP−/− (−/−) mice. The pictures were obtained by confocal imaging of neurons intracellularly labeled with neurobiotin. The pyramidal cells shown in STOP−/− were both injected. A total number of 27 STOP−/− CA1 pyramidal cells were examined and exhibit normal somatodendritic organization. Bars, A–E, 0.5 mm; F, 20 μm; G, 40 μm.

Article Snippet: The following drugs were used: NBQX, D-APV, DCGIV (Tocris), picrotoxin (Sigma), neurobiotin, and avidin Texas red D (Vector Laboratories, AbCys, France).

Techniques: Staining, Expressing, Activity Assay, Imaging, Labeling, Injection